Processed plasma is a great alternative to human serum for producing controls and calibrators, ensuring uniformity and consistency. It provides a base matrix for accurate analyte measurement and improves assay reliability.
Processed human plasma undergoes a variety of specialized techniques to address potential interferences and inconsistencies found in unprocessed plasma. These techniques ultimately lead to a more reliable solution for your research needs.
Our processing methods include defibrination, charcoal stripping, large-scale filtration or delipidation, and dialysis.
Defibrination
Defibrinated plasma is plasma from which fibrinogen and other clotting factors have been removed, preventing coagulation
Stable matrix for controls and calibrators, preventing coagulation from affecting assay performance
Charcoal Stripping
Charcoal stripping removes lipophilic substances like hormones and lipids from plasma by adsorbing them onto activated charcoal
Charcoal stripped plasma is free of lipophilic substances that may interfere with assays, enhancing specificity and accuracy, particularly in hormone and lipid assays
Filtration
Filtration eliminates particulate matter and aggregates from plasma, producing a clear and particle-free solution
0.1 µm filtered
Filtered plasma provides a clean matrix for controls and calibrators, ensuring reliable assay performance and improving diagnostic test accuracy and precision by removing interference from particulate matter
Delipidation
Delipidation is the process of removing lipids (fats) from plasma, which reduces lipid interference in assays
Delipidated plasma provides a clean matrix for controls and calibrators, reducing lipid interference in assays and ensuring accurate measurement of analyte
Dialysis
Eliminates the excess of calcium used in the defibrination step
It ensures more stable and normalized parameters
| Technique | Purpose | Use in calibrators and controls |
|---|---|---|
|
Defibrination |
Defibrinated plasma is plasma from which fibrinogen and other clotting factors have been removed, preventing coagulation |
Stable matrix for controls and calibrators, preventing coagulation from affecting assay performance |
|
Charcoal Stripping |
Charcoal stripping removes lipophilic substances like hormones and lipids from plasma by adsorbing them onto activated charcoal |
Charcoal stripped plasma is free of lipophilic substances that may interfere with assays, enhancing specificity and accuracy, particularly in hormone and lipid assays |
|
Filtration |
Filtration eliminates particulate matter and aggregates from plasma, producing a clear and particle-free solution |
Filtered plasma provides a clean matrix for controls and calibrators, ensuring reliable assay performance and improving diagnostic test accuracy and precision by removing interference from particulate matter |
|
Delipidation |
Delipidation is the process of removing lipids (fats) from plasma, which reduces lipid interference in assays |
Delipidated plasma provides a clean matrix for controls and calibrators, reducing lipid interference in assays and ensuring accurate measurement of analyte |
|
Dialysis |
Eliminates the excess of calcium used in the defibrination step |
It ensures more stable and normalized parameters |
We understand your research needs are unique. Our processed human plasma can be customized upon request. Feel free to reach out to us with your specific requirements.
Defibrinated plasma forms the foundation of our processed human plasma offerings. It's produced by separating plasma from whole blood and then removing fibrinogen, a clotting factor. This defibrination process creates a more consistent and homogeneous sample compared to off-the-clot (OTC) serum.
Defibrinated plasma is a versatile option ideal for researchers needing a well-characterized and consistent processed plasma solution for their assays.
Standard human serum (SHS) represents a further refinement of defibrinated plasma, offering the highest level of purity among our processed plasma options. It presents a reliable replacement for off-the-clot (OTC) serum.
SHS is produced by subjecting defibrinated plasma to a specialized high purification process that significantly lowers citrate levels from approximately 60 mg/dL to an average of 3 mg/dL, which is within the physiological range values.
SHS Advantages:
SHS is the optimal choice for researchers requiring a high level of purity, consistency, and minimal interference for their assays. Its exceptional characteristics make it ideal for sensitive assays and research demanding the most reliable data.
Selecting the right plasma is crucial for your research. This table compares three common options: off-the-clot serum (OTC serum), defibrinated plasma, and standard human serum. Each option has distinct characteristics due to its processing method (or lack thereof for OTC serum). Understanding these differences will help you choose the plasma most suited to your assay requirements.
Description
Serum collected from whole blood that is allowed to coagulate naturally
Plasma converted to serum by adding calcium and thrombin to generate the clot
Defibrinated plasma that undergoes a further high purification process that significantly reduces citrate concentration to physiological levels
Collection Method
Blood donation
Plasmapheresis
Plasmapheresis
Anticoagulant
No addition
Sodium citrate
Sodium citrate
Citrate Concentration
Physiological levels: 1.3-7.6 mg/dL¹
~60 mg/dL
~3 mg/dL
Collection per Donor
1.5 L/Year
83.2 L/Year
83.2 L/Year
Recommended Use
Negative base matrix
Standard use for large negative base matriz manufacturing
Use for large negative base matrix manufacturing when additive factors can interfere in the manufacturing process
| OTC Serum | Defibrinated Plasma (Processed Plasma) | Standard Human Serum (Processed Plasma) | |
|---|---|---|---|
|
Description |
Serum collected from whole blood that is allowed to coagulate naturally |
Plasma converted to serum by adding calcium and thrombin to generate the clot |
Defibrinated plasma that undergoes a further high purification process that significantly reduces citrate concentration to physiological levels |
|
Collection Method |
Blood donation |
Plasmapheresis |
Plasmapheresis |
|
Anticoagulant |
No addition |
Sodium citrate |
Sodium citrate |
|
Citrate Concentration |
Physiological levels: 1.3-7.6 mg/dL¹ |
~60 mg/dL |
~3 mg/dL |
|
Collection per Donor |
1.5 L/Year |
83.2 L/Year |
83.2 L/Year |
|
Recommended Use |
Negative base matrix |
Standard use for large negative base matriz manufacturing |
Use for large negative base matrix manufacturing when additive factors can interfere in the manufacturing process |
Quality Assurance and Safety
Our human serum products are collected at FDA- and EMA-licensed centers and manufactured under a strict quality assurance system, delivering quality and safety you can rely on.
Large Collection Capabilities
Grifols boasts the largest plasma donor center network worldwide, with over 350 centers strategically distributed across the US, Europe, and Africa. This extensive network facilitates over 45,000 daily donations, ensuring a consistent and reliable supply to meet your research needs no matter your location.
Expert Support
Our skilled customer service team is committed to providing outstanding support at every stage of the process.
Customization Solutions
Our manufacturing processes can be tailored to customer specifications.
Contact a Grifols representative today to get more information.
Human Male AB Serum
Blood-derived material collected from a male individual with AB blood type, suitable for various cell culture applications.
HumaCell Serum
HumaCell is a human serum ideal for culturing human cells, sourced for mixed gender and mixed blood types. This product is manufactured under cGMP.
Disease State Human Plasma and Serum
Human plasma and serum characterized for the presence or absence of disease-specific biomarkers (autoimmune diseases, infectious diseases, inflammatory or other conditions).
Human Serum
Human serum obtained from whole blood and allowed to coagulate naturally without anticoagulants.